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KMID : 0043319910140030255
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Archives of Pharmacal Research
1991 Volume.14 No. 3 p.255 ~ p.260
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Identification of Chitinolytic System in Allium fistulosum
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Kim YS
Lee EB/Joo SH
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Abstract
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Chitinase was partially purified from Allium fistulosum L. (green onion). Protein fraction precipitated from ammonium sulfate was passed through CM-Sepharose and Sephacryl HR-200. The specific activity of the chitinase was 6.4 units/mg and total recovery was 6.3%. The analysis of the products from the digestion of N-acetychitohexaose indicated that chitinase was endo in action, with oligerms from N-acetylchitobiose to chitotetraose. N-Acetylglucosaminidase from the same species hydrolyzed oligomers obtained from chitinase reaction to lower oligosaccharides. These data demonstrated that chitinolytic system exists in green onion.
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KEYWORD
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